全文获取类型
收费全文 | 3441篇 |
免费 | 546篇 |
国内免费 | 293篇 |
出版年
2023年 | 132篇 |
2022年 | 114篇 |
2021年 | 184篇 |
2020年 | 223篇 |
2019年 | 226篇 |
2018年 | 197篇 |
2017年 | 174篇 |
2016年 | 173篇 |
2015年 | 182篇 |
2014年 | 191篇 |
2013年 | 244篇 |
2012年 | 146篇 |
2011年 | 165篇 |
2010年 | 113篇 |
2009年 | 169篇 |
2008年 | 176篇 |
2007年 | 162篇 |
2006年 | 166篇 |
2005年 | 185篇 |
2004年 | 165篇 |
2003年 | 125篇 |
2002年 | 100篇 |
2001年 | 52篇 |
2000年 | 42篇 |
1999年 | 36篇 |
1998年 | 28篇 |
1997年 | 21篇 |
1996年 | 19篇 |
1995年 | 27篇 |
1994年 | 27篇 |
1993年 | 21篇 |
1992年 | 16篇 |
1991年 | 15篇 |
1990年 | 18篇 |
1989年 | 12篇 |
1988年 | 8篇 |
1987年 | 14篇 |
1986年 | 8篇 |
1985年 | 24篇 |
1984年 | 17篇 |
1983年 | 15篇 |
1982年 | 17篇 |
1981年 | 20篇 |
1980年 | 15篇 |
1979年 | 15篇 |
1978年 | 26篇 |
1977年 | 18篇 |
1976年 | 11篇 |
1975年 | 11篇 |
1974年 | 5篇 |
排序方式: 共有4280条查询结果,搜索用时 15 毫秒
1.
2.
The nocturnally active weakly electric fish Gnathonemus petersii is known to employ active electrolocation for the detection of objects and for orientation in its environment. The fish emits pulse‐type electric signals with an electric organ and perceives these signals with more than 3,000 epidermal electroreceptor organs, the mormyromasts, which are distributed over the animal's skin surface. In this study, we measured the metric dimensions of the mormyromasts from different body regions to find structural and functional specialization of the various body parts. We focused on the two foveal regions of G. petersii, which are located at the elongated and movable chin (the Schnauzenorgan; SO) and at the nasal region (NR), the skin region between the mouth and the nares. These two foveal regions were compared to the dorsal part (back) of the fish, which contains typical nonfoveal mormyromasts. While the gross anatomy of the mormyromasts from all skin regions is similar, the metric dimensions of the main substructures differed. The mormyromasts at the SO are the smallest and contain the smallest receptor cells. In addition, the number of receptor cells per organ is lowest at the SO. In contrast, at the back the biggest receptor organs with the highest amount of receptor cells per organ occur. The mormyromasts at the NR are in several respects intermediate between those from the back and the SO. However, mormyromasts at the NR are longer than those at all other skin regions, the canal leading from the receptor pore to the inner chambers were the longest and the overlaying epidermal layers are the thickest. These results show that mormyromasts and the epidermis they are embedded in at both foveal regions differ specifically from those found on the rest of the body. The morphological specializations lead to functional specialization of the two foveae. J. Morphol., 2012. © 2012 Wiley Periodicals, Inc. 相似文献
3.
《Cell》2022,185(17):3201-3213.e19
4.
5.
6.
《Journal of molecular biology》2021,433(3):166756
The pathogens Vibrio cholerae and Haemophilus influenzae use tripartite ATP-independent periplasmic transporters (TRAPs) to scavenge sialic acid from host tissues. They use it as a nutrient or to evade the innate immune system by sialylating surface lipopolysaccharides. An essential component of TRAP transporters is a periplasmic substrate binding protein (SBP). Without substrate, the SBP has been proposed to rest in an open-state, which is not recognised by the transporter. Substrate binding induces a conformational change of the SBP and it is thought that this closed state is recognised by the transporter, triggering substrate translocation. Here we use real time single molecule FRET experiments and crystallography to investigate the open- to closed-state transition of VcSiaP, the SBP of the sialic acid TRAP transporter from V. cholerae. We show that the conformational switching of VcSiaP is strictly substrate induced, confirming an important aspect of the proposed transport mechanism. Two new crystal structures of VcSiaP provide insights into the closing mechanism. While the first structure contains the natural ligand, sialic acid, the second structure contains an artificial peptide in the sialic acid binding site. Together, the two structures suggest that the ligand itself stabilises the closed state and that SBP closure is triggered by physically bridging the gap between the two lobes of the SBP. Finally, we demonstrate that the affinity for the artificial peptide substrate can be substantially increased by varying its amino acid sequence and by this, serve as a starting point for the development of peptide-based inhibitors of TRAP transporters. 相似文献
7.
《Critical reviews in biochemistry and molecular biology》2013,48(5):409-435
Telomere DNA-binding proteins protect the ends of chromosomes in eukaryotes. A subset of these proteins are constructed with one or more OB folds and bind with G+T-rich single-stranded DNA found at the extreme termini. The resulting DNA-OB protein complex interacts with other telomere components to coordinate critical telomere functions of DNA protection and DNA synthesis. While the first crystal and NMR structures readily explained protection of telomere ends, the picture of how single-stranded DNA becomes available to serve as primer and template for synthesis of new telomere DNA is only recently coming into focus. New structures of telomere OB fold proteins alongside insights from genetic and biochemical experiments have made significant contributions towards understanding how protein-binding OB proteins collaborate with DNA-binding OB proteins to recruit telomerase and DNA polymerase for telomere homeostasis. This review surveys telomere OB protein structures alongside highly comparable structures derived from replication protein A (RPA) components, with the goal of providing a molecular context for understanding telomere OB protein evolution and mechanism of action in protection and synthesis of telomere DNA. 相似文献
8.
9.
《Cell reports》2020,30(4):1152-1163.e4
10.
Zaki Monawar Eisa 《Saudi Journal of Biological Sciences》2011,18(2):195-200
Hepatitis C virus (HCV) causes serious infections in the liver which may lead to liver cirrhosis and hepatocellular carcinoma. Non structural 3 (NS3) protein is one of the most important proteins of the virus which has protease and helicase activities. Protease activity has a crucial role in the replication and persistence of the virus. Site directed mutation was carried out in the protease region of one NS3 and another site directed mutation in the helicase region of another NS3. The expression of both mutated NS3 was compared with wild NS3. Expression of the three different NS3 types was confirmed by in situ staining and western blotting using an anti-NS3 antibody and correlated with a reduced antiviral response after treatment with interferon-α. Mutation analysis showed that the NS3 protease activity andnot the NS3 helicase was essential for the inhibition of the interferon-α response. 相似文献